Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay
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چکیده
منابع مشابه
Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay
Description of the technology Elispot is a workhorse for functional analysis of single immune cells. Originally established for the enumeration of specific antibody-secreting B cells in 1983 [1], the technology has been expanded to the analysis of T cells, NK cells and monocytes. The assay is typically carried out in 96 well plates with a nitrocellulose or PVDF membrane, which is coated with an...
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Adaptive immunity is an important component to clearance of intracellular pathogens. The ability to detect and quantify these responses in humans is an important diagnostic tool. The enzyme-linked immunospot assay (ELISPOT) is gaining popularity for its ability to identify cellular immune responses against microbial antigens, including immunosuppressed populations such as those with HIV infecti...
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BACKGROUND Recent biotechnological developments have resulted in increasing interest in immunology biomarkers. These biomarkers have potential clinical utility in the near future as predictors of treatment response. Hence, clinical validation of these predictive markers is critical. FINDINGS The process of clinically validating a predictive biomarker is reviewed. Validation of a predictive bi...
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Abbreviations: CC, Colon cancer; CRC, Colorectal cancer; CT, Core of the tumor; DAB, Diaminobenzidine; DFS, Disease-free-survival; DP, Digital pathology; DSS, Disease-specific-survival; FFPE, Formalin-fixed paraffin-embedded; IHC, Immunohistochemistry; IM, Invasive margin; INSERM, Institut national de la santé et de la recherche médicale; IS, Immunoscore; IVD, In vitro diagnostic; MSI, Microsat...
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ژورنال
عنوان ژورنال: Journal for Immunotherapy of Cancer
سال: 2015
ISSN: 2051-1426
DOI: 10.1186/s40425-015-0074-0